CD16 antibody

Catalog No. ABIN319692

This anti-CD16 antibody is a Mouse Monoclonal antibody detecting CD16 in FACS and IP. Suitable for Human.

Quick Overview for CD16 antibody (ABIN319692)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: LNK16

Product Details anti-CD16 Antibody

Specificity: This antibody recognizes CD16. This antibody has been reported to block the binding of IgG to the CD16 Molecule. (4)

Purification: Ion Exchange Chromatography

Immunogen: Normal Human peripheral blood granulocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Use 10 μL of 1/10-1/20 diluted antibody to label 10^6 cells or 100 μL human whole blood. Immunoprecipitation. Does not work in Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: CD16 is a 50-65kD cell surface molecule which exists in two forms, a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID: 2214

NCBI Accession: NP_000560

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process