AOX1/AOX2 antibody (C-Term)

Catalog No. ABIN3197483

This anti-AOX1/AOX2 antibody is a Rabbit Polyclonal antibody detecting AOX1/AOX2 in WB. Suitable for Arabidopsis thaliana, Lupinus luteus, Moss, Nicotiana tabacum, Pisum sativum and Potato. This Primary Antibody has been cited in 7+ publications.

Quick Overview for AOX1/AOX2 antibody (C-Term) (ABIN3197483)

Target: AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))

Reactivity: Arabidopsis thaliana, Lupinus luteus, Moss, Nicotiana tabacum, Pisum sativum, Potato

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-AOX1/AOX2 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): No cross-reactivity with: Chlamydomonas reinhardtii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 36-40 / 36-40 for Arabidopsis thaliana

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thaliana AOX1A (At3g22370) and AOX2 (At3g64210), Solanum lycopersicum Q7XBG9, Oryza sativa Q7XT33,

Application Details

Application Notes: Recommended Dilution: 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-AOX1/AOX2 antibody (ABIN3197483)

Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

Tsaniklidis, Delis, Nikoloudakis, Katinakis, Aivalakis: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).

Zubo, Potapova, Yamburenko, Tarasenko, Konstantinov, Börner: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

Kornfeld, Heskel, Atkin, Gough, Griffin, Horton, Turnbull: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Wang, Li, Liu, He, Bi: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).

Target Details for AOX1/AOX2

Target: AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))

Alternative Name: Plant alternative oxidase 1 and 2 (AOX1/2)

Background: AGI Code: At3g22370
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.

Molecular Weight: expected: 36-40 kDa, apparent: 36-40 for Arabidopsis thaliana

UniProt: Q7XBG9, Q7XT33, O22049, Q39219