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Tubulin alpha Chain (TUB1) antibody

This Rabbit Polyclonal antibody specifically detects in IF and WB. It exhibits reactivity toward Arabidopsis thaliana, Barley and Zea mays.
Catalog No. ABIN7477855

Quick Overview for Tubulin alpha Chain (TUB1) antibody (ABIN7477855)

Target

Tubulin alpha Chain (TUB1)

Reactivity

Arabidopsis thaliana, Barley, Zea mays

Host

Rabbit

Clonality

Polyclonal

Application

Immunofluorescence (IF), Western Blotting (WB)
  • Cross-Reactivity

    Arabidopsis thaliana, Barley (Hordeum vulgare), Maize/Corn (Zea mays)

    Cross-Reactivity (Details)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    Predicted Reactivity

    dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Triticum aestivum, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, algae: Chlorella vulgaris, Euglena gracilis, Micromonoas pusilla, Ostreococcus lucimarinus

    Characteristics

    Expected / apparent Molecular Weight of the Antigene: 49 / 52 kDa (Arabidopsis thaliana)
    Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

    Purification

    serum

    Immunogen

    KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139(At1g64740), alpha-2/alpha-4 chain B9DGT7(At1g50010), alpha-5 chain B9DHQ0(At5g19780), alpha-6-chain P29511(At4g14960)
  • Application Notes

    1: 1000 with standard ECL (WB), 1: 500 (IF)

    Comment

    Peptide used to elict this antibody is not present in tubulin beta.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 100 μL of sterile water

    Storage

    -20 °C

    Storage Comment

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target

    Tubulin alpha Chain (TUB1)

    Alternative Name

    Tubulin alpha chain

    Background

    AGI Code: At1g64740
    Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

    Molecular Weight

    expected: 49 kDa, apparent: 52 kDa (Arabidopsis thaliana)

    UniProt

    P11139, P29510, P20363, P29511
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