CD31 antibody (AA 199-287)

Catalog No. ABIN3201382

The Mouse Monoclonal anti-CD31 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CD31 in samples from Human.

Quick Overview for CD31 antibody (AA 199-287) (ABIN3201382)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 15#

Product Details anti-CD31 Antibody

Binding Specificity: AA 199-287

Purpose: Monoclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)

Sequence: MGHHHHHHSG SEF- DR VLSFRCQARI ISGIHMQTSE STKSELVTVT ESFSTPKFHI SPTGMIMEGA QLHIKCTIQV THLAQEFPEI IIQKDKAIVA HNRHGNK

Specificity: The antibody is a mouse monoclonal antibody raised against PECAM1. It has been selected for its ability to recognize PECAM1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1) corresdonding to Asp199~Lys287 (Accession # P16284)

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1

Background: CD31, EndoCAM, GPIIA', PECA1

Pathways: Regulation of Actin Filament Polymerization