CD52 antibody

Catalog No. ABIN320174

The Rat Monoclonal anti-CD52 antibody is suitable to detect CD52 in samples from Human and Monkey. It has been validated for FACS, WB, IP, IHC (fro), CyTox and EIA.

Quick Overview for CD52 antibody (ABIN320174)

Target: CD52

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD52 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Enzyme Immunoassay (EIA)

Clone: YTH34-5

Product Details anti-CD52 Antibody

Specificity: This antibody reacts with CD52 antigen. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.

Purification: Affinity Chromatography on Protein A .

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (1/20): Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/20). Immunohistochemistry on Paraffin Sections (1/20-1/40). Immunohistochemistry on Resin. Positive Control Tissue: Tonsil. ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS 0.09 % containing Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1

Background: CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

NCBI Accession: NP_001794

UniProt: P31358