CD52 antibody

Catalog No. ABIN320174

Product Details anti-CD52 Antibody

Target: CD52

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD52 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with CD52 antigen. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.

Purification: Affinity Chromatography on Protein A .

Clone: YTH34-5

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (1/20): Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/20). Immunohistochemistry on Paraffin Sections (1/20-1/40). Immunohistochemistry on Resin. Positive Control Tissue: Tonsil. ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS 0.09 % containing Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1 (CD52 Products)

Background: CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

NCBI Accession: NP_001794

UniProt: P31358