CD31 antibody (APC)

Catalog No. ABIN320179

This Mouse Monoclonal antibody specifically detects CD31 in FACS. It exhibits reactivity toward Human and Pig.

Quick Overview for CD31 antibody (APC) (ABIN320179)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: LCI-4

Product Details anti-CD31 Antibody (APC)

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Porcine CD31/human IgGFc fusion protein.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (Neat-1/10): Use 10 μL of the suggested working dilution to label 100 μL of whole blood or 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 / PECAM1

Background: Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms' tumors, Ewing's sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM Integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66 % of patients with Kallmann's syndrome, anosmia with secondary hypogonadism.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

Gene ID: 9606

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization