CD8 antibody (AA 3-63) (FITC)

Catalog No. ABIN320351

The Mouse Monoclonal anti-CD8 antibody has been validated for FACS. It is suitable to detect CD8 in samples from Pig.

Quick Overview for CD8 antibody (AA 3-63) (FITC) (ABIN320351)

Target: CD8

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MIL-12

Product Details anti-CD8 Antibody (FITC)

Binding Specificity: AA 3-63

Specificity: This antibody recognises a subset of T cells.

Purification: Affinity Chromatography on Protein G

Immunogen: Porcine mesenteric lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 396627

UniProt: P01732