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ALT antibody (AA 115-164)

This Rabbit Polyclonal antibody specifically detects ALT in WB. It exhibits reactivity toward Human, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Hamster and Monkey.
Catalog No. ABIN320980

Quick Overview for ALT antibody (AA 115-164) (ABIN320980)

Target

See all ALT Antibodies
ALT (Alanine Aminotransferase (ALT))

Reactivity

  • 74
  • 35
  • 24
  • 18
  • 7
  • 6
  • 5
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Hamster, Monkey

Host

  • 92
  • 33
  • 5
  • 1
Rabbit

Clonality

  • 95
  • 35
Polyclonal

Conjugate

  • 87
  • 22
  • 14
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ALT antibody is un-conjugated

Application

  • 117
  • 82
  • 59
  • 47
  • 27
  • 14
  • 8
  • 8
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
Western Blotting (WB)
  • Binding Specificity

    • 11
    • 10
    • 8
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 115-164

    Specificity

    Human GPT / ALT1

    Predicted Reactivity

    Percent identity by BLAST analysis: Mouse, Pig, Guinea pig (92%) Dog (85%).

    Purification

    Protein A purified

    Immunogen

    Synthetic peptide located between aa115-164 of human GPT (P24298, NP_005300). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Hamster (100%), Gorilla, Galago, Mouse, Rat, Bovine, Pig, Guinea pig (92%), Elephant, Dog, Bat (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute with 100 μL sterile ddH2O

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    ALT (Alanine Aminotransferase (ALT))

    Alternative Name

    GPT / Alanine Transaminase

    Background

    Name/Gene ID: GPT

    Synonyms: GPT, AAT1, ALT1, Alanine aminotransferase, Alanine aminotransferase 1, GPT 1, GPT1

    Gene ID

    2875

    NCBI Accession

    NP_005300

    UniProt

    P24298
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