PPIF antibody (AA 92-141)

Catalog No. ABIN321058

This Rabbit Polyclonal antibody specifically detects PPIF in WB. It exhibits reactivity toward Human, Rat, Mouse, Rabbit, Dog, Cow, Guinea Pig, Horse, Bat, Chicken, Hamster, Pig and Xenopus laevis.

Quick Overview for PPIF antibody (AA 92-141) (ABIN321058)

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Reactivity: Human, Rat, Mouse, Rabbit, Dog, Cow, Guinea Pig, Horse, Bat, Chicken, Hamster, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPIF Antibody

Binding Specificity: AA 92-141

Specificity: Human PPIF / Cyclophilin F

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken (100%) Zebrafish (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa92-141 of human PPIF (P30405, NP_005720). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%), Gorilla, Marmoset, Platypus, Frog, Catfish, Salmon, Smelt, Stickleback, Pike, Zebrafish (92%), Blood fluke (91%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPIF

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Alternative Name: PPIF / Cyclophilin F

Background: Name/Gene ID: PPIF

Synonyms: PPIF, Cyclophilin 3, CYP3, Cyp-D, HCyP3, Peptidylprolyl isomerase F, Rotamase F, PPIase F, Cyclophilin D, Cyclophilin F

Gene ID: 10105

NCBI Accession: NP_005720

UniProt: P30405

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity