Carboxypeptidase A2 antibody (AA 17-417)

Catalog No. ABIN322591

This Mouse Monoclonal antibody specifically detects Carboxypeptidase A2 in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for Carboxypeptidase A2 antibody (AA 17-417) (ABIN322591)

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Carboxypeptidase A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Carboxypeptidase A2 Antibody

Binding Specificity: AA 17-417

Specificity: Recognizes human CPA2. Recognizes the pro form, (aa 17 - 417) but not the activated enzyme (aa 113 - 417) in Western blots. Does not cross-react with rhCPA1, rhCPA4, rhCPB1 or rhCPE. Species sequence homology: chimpanzee 99.5 % , canine 90 %, rat, 87 %, mouse 86 % and chicken 70 %.

Purification: Protein G purified

Immunogen: NS0-derived recombinant human rhCPA2.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western blot, Immunoprecipitation, and Direct ELISA. Western blot: 1-2 μg/mL Using a colorimetric detection system, the detection limit for rhCPA2 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL. Direct ELISA: 0.5-1.0 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute, sterile PBS.

Concentration: Lot specific

Buffer: PBS, 5 % trehalose. Reconstitute, sterile PBS.

Target Details for Carboxypeptidase A2

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Alternative Name: CPA2

Background: Name/Gene ID: CPA2
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPA2, Carboxypeptidase A2

Gene ID: 1358

UniProt: P48052