IL17F antibody (AA 31-163)

Catalog No. ABIN327416

The Mouse Monoclonal anti-IL17F antibody has been validated for ELISA and FACS. It is suitable to detect IL17F in samples from Human.

Quick Overview for IL17F antibody (AA 31-163) (ABIN327416)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL17F antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: 197301

Product Details anti-IL17F Antibody

Binding Specificity: AA 31-163

Specificity: Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.

Purification: Protein G purified

Immunogen: E. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 μg/mL. Add 10 μL to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 μg/mL. The detection limit is ~1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL17F

Background: Name/Gene ID: IL17F

Synonyms: IL17F, CANDF6, Cytokine ML-1, IL-17F, Interleukin-17F, Interleukin 17F, Interleukin-24, ML-1, ML1, Mutant IL-17F

Gene ID: 112744

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity