EMP3 antibody

Catalog No. ABIN329188

Product Details anti-EMP3 Antibody

Target: EMP3 (Epithelial Membrane Protein 3 (EMP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EMP3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Recognizes human EMP3 cell transfectants but not irrelevant transfectants.

Purification: Protein G purified

Immunogen: NS0 cells transfected with human EMP3.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo

Usage: Suitable for use in Flow Cytometry. Flow Cytometry: This antibody was validated for flow cytometry using human PBMC. For intracellular staining to detect EMP3, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 0.2 mL sterile 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.2 mL sterile 40-50 % glycerol, PBS.

Target Details for EMP3

Target: EMP3 (Epithelial Membrane Protein 3 (EMP3))

Alternative Name: EMP3 (EMP3 Products)

Background: Name/Gene ID: EMP3

Synonyms: EMP3, EMP-3, Epithelial membrane protein 3, HNMP-1, Protein YMP, YMP

Gene ID: 2014

UniProt: P54852