EMP3 antibody
Quick Overview for EMP3 antibody (ABIN329188)
Target
See all EMP3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Recognizes human EMP3 cell transfectants but not irrelevant transfectants.
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Purification
- Protein G purified
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Immunogen
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NS0 cells transfected with human EMP3.
Type of Immunogen: Cells -
Isotype
- IgG2b
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Application Notes
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Approved: Flo
Usage: Suitable for use in Flow Cytometry. Flow Cytometry: This antibody was validated for flow cytometry using human PBMC. For intracellular staining to detect EMP3, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute, 0.2 mL sterile 40-50 % glycerol, PBS.
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Concentration
- Lot specific
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Buffer
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.2 mL sterile 40-50 % glycerol, PBS.
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- EMP3 (Epithelial Membrane Protein 3 (EMP3))
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Alternative Name
- EMP3
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Background
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Name/Gene ID: EMP3
Synonyms: EMP3, EMP-3, Epithelial membrane protein 3, HNMP-1, Protein YMP, YMP -
Gene ID
- 2014
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UniProt
- P54852
Target
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