FOXH1 antibody (AA 164-272)

Catalog No. ABIN329971

This Sheep Polyclonal antibody specifically detects FOXH1 in WB, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for FOXH1 antibody (AA 164-272) (ABIN329971)

Target: FOXH1 (Forkhead Box H1 (FOXH1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This FOXH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-FOXH1 Antibody

Binding Specificity: AA 164-272

Specificity: Recognizes human FoxH1. Species sequence homology: mouse 55 % . less than 1 % cross-reactivity with rhFoxB2.

Purification: Affinity purified

Immunogen: E. coli-derived recombinant human rhFoxH1 (aa 164 - 272).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western blot, Immunocytochemistry and Direct ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.2 ng/well. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1. (rhFoxH1, aa 164-272, Accession # O75593).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 500 μL sterile PBS to a concentration of 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 500 μLsterile PBS to a concentration of 0.2 mg/mL.

Target Details for FOXH1

Target: FOXH1 (Forkhead Box H1 (FOXH1))

Alternative Name: FOXH1

Background: Name/Gene ID: FOXH1
Family: Transcription factor

Synonyms: FOXH1, Fast-2, FAST2, FAST-1, FAST1, Forkhead box H1, Forkhead box protein H1, HFAST-1

Gene ID: 8928

UniProt: O75593

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling