Actin antibody (N-Term)

Catalog No. ABIN331838

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC, ELISA, IHC (p) and ICC. It is suitable to detect Actin in samples from Human, Rat, Mouse, Chicken, Rabbit, Guinea Pig and Sheep.

Quick Overview for Actin antibody (N-Term) (ABIN331838)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Guinea Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Actin Antibody

Binding Specificity: AA 50-70, N-Term

Specificity: Recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species cross-reactivity: All species.

Purification: Protein A purified

Immunogen: Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.

Type of Immunogen: Purified protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, ICC (1 - 20 μg/mL), IHC, IHC-P, WB

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Immunohistochemistry: 10 μg/mL (paraffin embedded, 4 % formaldehyde, 3 % glutaraldehyde, sodium cacodylate treated sections). Visualized using a donkey anti-mouse IgG (Cy3). Positive staining pattern reflects both membrane and cytoplasmic staining. ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoideum and Physarum polycephalum in ELISA.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Actin

Target: Actin