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DS WB Results: Approx 18 kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7 kDa according to NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:8000.