ADP-Ribosylation Factor 1 (ARF1) (C-Term) antibody

Details for Product No. ABIN334352
Target
Binding Specificity
C-Term
10
10
9
7
7
6
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
94
44
41
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Goat
74
21
Clonality
Polyclonal
Conjugate
Un-conjugated
8
7
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
62
42
42
17
13
12
12
8
6
5
3
3
2
2
2
1
Options
Purpose ARF1 (variants 1-4)
Immunogen Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1.
Blocking Peptide Blocking peptide for this product available: ABIN368983
Sequence EGLDWLSNQL RNQK
Isotype IgG
Specificity This antibody is expected to recognize all four reported isoforms.
Predicted Reactivity Human, Mouse, Rat, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name ARF1 (ARF1 Antibody Abstract)
Background ARF1, ARF 1, ADP-ribosylation factor 1, ADP ribosylation factor 1
Gene ID 375, 376, 377
NCBI Accession NP_001019397, NP_001649, NP_001019398, NP_001019399
Pathways Transition Metal Ion Homeostasis, Inositol Metabolic Process
Application Notes

DS WB Results: Approx 18 kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7 kDa according to NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-ADP-Ribosylation Factor 1 (ARF1) (C-Term) antibody (ABIN334352) ABIN334352 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Dete...