ARF1 antibody (C-Term)

Catalog No. ABIN6939844

The Mouse Monoclonal anti-ARF1 antibody is suitable to detect ARF1 in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for ARF1 antibody (C-Term) (ABIN6939844)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 1A9-5

Product Details anti-ARF1 Antibody

Binding Specificity: C-Term

Purpose: ARF1 (Golgi Apparatus Marker) Antibody

Specificity: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60 % sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: C-terminal human ARF1 peptide SNQLRNQ

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Kidney, Liver, Brain or Breast.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background:

ADP-ribosylation factor 1, ARF1

Brain or Breast., HeLa cells. Kidney, Liver

Molecular Weight: 20kDa

Gene ID: 375, 286221

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process