ARF1 antibody

Catalog No. ABIN6939846

This Mouse Monoclonal antibody specifically detects ARF1 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Rabbit, Guinea Pig and Hamster.

Quick Overview for ARF1 antibody (ABIN6939846)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Rat, Mouse, Rabbit, Guinea Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ARF1-2117

Product Details anti-ARF1 Antibody

Purpose: ARF1 (Golgi Apparatus Marker) Antibody

Specificity: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60 % sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human ARF1 protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Kidney, Liver, Brain or Breast.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background:

ADP-ribosylation factor 1, ARF1

Brain or Breast., HeLa cells. Kidney, Liver, Pancreas or Lung

Molecular Weight: 20kDa

Gene ID: 375, 286221

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process