BIN1 antibody (Internal Region)

Catalog No. ABIN334360

This Goat Polyclonal antibody specifically detects BIN1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for BIN1 antibody (Internal Region) (ABIN334360)

Target: BIN1 (Bridging Integrator 1 (BIN1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BIN1 Antibody

Binding Specificity: Internal Region

Purpose: BIN1

Sequence: SDNAPAKGNK SPS

Specificity: This antibody is expected to recognise all ten reported isoforms (NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_004296.1, NP_647600.1, NP_647601.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SDNAPAKGNKSPS, from the internal region of the protein sequence according to NP_004296.1, NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_647600.1, NP_647601.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Experiments gave bands at approx 70 kDa and 60 kDa in Human Skeletal Muscle lysates after 0.1 μg/mL antibody staining. These bands correspond to earlier findings in literature with different antibodies (Chang et al, Cancer Res. 2007 Jan 1,67(1

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BIN1

Target: BIN1 (Bridging Integrator 1 (BIN1))

Alternative Name: BIN1

Background: BIN1, bridging integrator 1, AMPH2, AMPHL, DKFZp547F068, MGC10367, SH3P9, amphiphysin II, amphiphysin-like, box dependant MYC interacting protein 1

Gene ID: 274, 30948, 117028

NCBI Accession: NP_004296, NP_647593, NP_647594, NP_647595, NP_647596, NP_647597, NP_647598, NP_647599, NP_647600, NP_647601