COX IV antibody (C-Term)

Catalog No. ABIN334378

This Goat Polyclonal antibody specifically detects COX IV in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for COX IV antibody (C-Term) (ABIN334378)

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-COX IV Antibody

Binding Specificity: C-Term

Purpose: COX4I1

Sequence: QGLASKWDYE KNE

Specificity: This antibody is expected to recognize reported isoforms 1 and 4 (NP_001852.1, NP_001305726.1). Reported variants represent identical protein: NP_001305715.1, NP_001852.1. This product may cross-react to COX4I2 (GeneID 84701).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QGLASKWDYEKNE, from the C Terminus of the protein sequence according to NP_001852.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid and Testis. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 18 kDa band observed in human skeletal muscle lysates (calculated MW of 19.6 kDa according to NP_001852.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COX4I1

Background: COX4I1, cytochrome c oxidase subunit IV isoform 1 , COX4, COXIV, MGC72016

Gene ID: 1327

NCBI Accession: NP_001852, NP_001305726

Pathways: Proton Transport