FTL antibody (C-Term)

Catalog No. ABIN334402

This Goat Polyclonal antibody specifically detects FTL in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FTL antibody (C-Term) (ABIN334402)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FTL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-FTL Antibody

Binding Specificity: C-Term

Purpose: FTL

Sequence: GEYLFERLTL KHD

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GEYLFERLTLKHD, from the C Terminus of the protein sequence according to NP_000137.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx. 20 kDa band observed in Human Brain (Cerebellum), Liver, Placenta and 22 kDa in Mouse and Rat Brain lysates (calculated MW of 20.0 kDa according to NP_000137.2). Recommended concentration: 0.3-1.0 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: FTL

Background: FTL, ferritin, light polypeptide, MGC71996, L apoferritin, ferritin L subunit, ferritin L-chain, ferritin light chain, ferritin light polypeptide-like 3

Gene ID: 2512, 14325, 29292

NCBI Accession: NP_000137

Pathways: Transition Metal Ion Homeostasis