HAP1 antibody (Internal Region)

Catalog No. ABIN334416

This anti-HAP1 antibody is a Goat Polyclonal antibody detecting HAP1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for HAP1 antibody (Internal Region) (ABIN334416)

Target: HAP1 (Huntingtin Associated Protein 1 (HAP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-HAP1 Antibody

Binding Specificity: Internal Region

Purpose: HAP1

Sequence: RYDFRYSEDR EQ

Specificity: This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RYDFRYSEDREQ, from the internal region of the protein sequence according to NP_003940.2, NP_817084.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 70 kDa band observed in Human Brain (Frontal Cortex and Hippocampus) lysates (calculated MW of 69.6 kDa according to NP_817084.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band of unknown i

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HAP1

Target: HAP1 (Huntingtin Associated Protein 1 (HAP1))

Alternative Name: HAP1

Background: HAP1, huntingtin-associated protein 1 (neuroan 1), HAP2, HIP5, HLP, hHLP1, huntingtin-associated protein 1, huntingtin-associated protein 2, neuroan 1

Gene ID: 9001

NCBI Accession: NP_003940, NP_817084

Pathways: Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus