ATP Synthase Subunit gamma (AtpC) antibody

Catalog No. ABIN334563

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB. Suitable for Arabidopsis thaliana and Chlamydomonas reinhardtii.

Quick Overview for ATP Synthase Subunit gamma (AtpC) antibody (ABIN334563)

Target: ATP Synthase Subunit gamma (AtpC)

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Glycine max, Lens culinaris, Nicotiana tabacum, Physcomitrella patens, Pisum sativum, Populus jackii, Vitis vinifera, cyanobacteria

Characteristics: Expected / apparent Molecular Weight of the Antigene: 42 kDa (Chlamydomonas reinhardtii), 38 kDa (Spinacia oleracea)

Purification: serum

Immunogen: synthetic peptides derived from Arabidopsis thalina chloroplast localized ATP synthase subunit gamma chain 1 and 2 protein sequence ( At4g04640 and At1g15700) and Chlamydomonas reinhardtii ATP synthase subunit gamma protein sequence ( A8HXL8) coupled to KLH

Application Details

Application Notes: 1: 10 000 (WB), 1: 1000 (ELISA)

Comment:

apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: ATP Synthase Subunit gamma (AtpC)

Alternative Name: Gamma subunit of ATP synthase (AtpC)

Background: AGI Code: At4g04640
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.

Molecular Weight: expected: 35.3 , 42 (Chlamydomonas reinhardtii)35.6 kDa, apparent: 38 (Spinacia oleracea)

UniProt: Q01908, Q01909

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process