HSP70 antibody (Cytoplasmic)

Catalog No. ABIN334584

This anti-HSP70 antibody is a Rabbit Polyclonal antibody detecting HSP70 in WB and IP. Suitable for Plasmodium falciparum, Arabidopsis thaliana, Zea mays, Tomato, Barley and Algae (Desmodesmus subspicatus). This Primary Antibody has been cited in 4+ publications.

Quick Overview for HSP70 antibody (Cytoplasmic) (ABIN334584)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Plasmodium falciparum, Arabidopsis thaliana, Zea mays, Tomato, Barley, Algae (Desmodesmus subspicatus)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-HSP70 Antibody

Binding Specificity: Cytoplasmic

Characteristics: Expected / apparent Molecular Weight of the Antigene: 70 kDa
Detection limit is 4 pg, 11 fmol, linear range of logit/log plot is 0.02-6 pmol,midrange (B/ B o =50 %) 112 pg, 300 fmol.

Purification: serum

Immunogen: KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana At3g12580/T2E22_110 and At3g16030 and At3g12580/T2E22_110

Application Details

Application Notes: Recommended Dilution: 1 : 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extractof concentration 3-5 mg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 150 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-HSP70 antibody (ABIN334584)

Norhayati, Hazlina, Asrenee, Emilin: "Magnitude and risk factors for postpartum symptoms: a literature review." in: Journal of affective disorders, Vol. 175, pp. 34-52, (2015) (PubMed).

Muralidharan, Oksman, Pal, Lindquist, Goldberg: "Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers." in: Nature communications, Vol. 3, pp. 1310, (2012) (PubMed).

Alvarez, del Hoyo, García-Breijo, Reig-Armiñana, del Campo, Guéra, Barreno, Casano: "Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea." in: Journal of plant physiology, Vol. 169, Issue 18, pp. 1797-806, (2012) (PubMed).

Janicka-Russak, Kaba?a, Burzynski: "Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots." in: Journal of experimental botany, Vol. 63, Issue 11, pp. 4133-42, (2012) (PubMed).

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70

Background: AGI Code: At3g12580
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Molecular Weight: 70 kDa

NCBI Accession: NP_187555, NP_195869

UniProt: Q9LHA8