GNAT3 antibody (Internal Region)

Catalog No. ABIN335139

This anti-GNAT3 antibody is a Goat Polyclonal antibody detecting GNAT3 in ELISA and IHC. Suitable for Human.

Quick Overview for GNAT3 antibody (Internal Region) (ABIN335139)

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GNAT3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GNAT3 Antibody

Binding Specificity: Internal Region

Purpose: GNAT3

Sequence: KNQFLDLNLK KEDKE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration: 3-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 27 kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GNAT3

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Alternative Name: GNAT3

Background: GNAT3, guanine nucleotide binding protein, alpha transducing 3 , tcag7.1053, GDCA, gustducin alpha-3

Gene ID: 346562, 242851, 286924

NCBI Accession: NP_001095856

Pathways: Peptide Hormone Metabolism, G-protein mediated Events, Phototransduction