Lamin B1 antibody

Catalog No. ABIN335391

Cited in 9+ publications. This anti-Lamin B1 antibody is a Mouse Monoclonal antibody detecting Lamin B1 in WB, ICC and FACS. Suitable for Human, Mouse, Rat, Cow, Rabbit, Dog, Sheep and Zebrafish (Danio rerio).

Quick Overview for Lamin B1 antibody (ABIN335391)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Sheep, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 119D5-F1

Product Details anti-Lamin B1 Antibody

Specificity: Human, rat, mouse, bovine, rabbit, dog, sheep.

Purification: Purified

Immunogen: 119D5-F1 is a mouse monoclonal IgG1/kappa antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.

Isotype: IgG1

Application Details

Application Notes: 119D5-F1 reacts with an epitope located C-terminal of residue 231 in lamin B1. 119D5-F1 is suitable for immunocytochemistry, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Lamin B1 antibody (ABIN335391)

Broers, Ramaekers, Bonne, Yaou, Hutchison: "Nuclear lamins: laminopathies and their role in premature ageing." in: Physiological reviews, Vol. 86, Issue 3, pp. 967-1008, (2006) (PubMed).

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, (2003) (PubMed).

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, (1999) (PubMed).

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, (1997) (PubMed).

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, (1997) (PubMed).

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, (1997) (PubMed).

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, (1997) (PubMed).

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, (1996) (PubMed).

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, (1996) (PubMed).

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin B1

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Pathways: Apoptosis, Caspase Cascade in Apoptosis