Lamin B1 antibody

Catalog No. ABIN335391

Product Details anti-Lamin B1 Antibody

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Specificity: Human, rat, mouse, bovine, rabbit, dog, sheep.

Purification: Purified

Immunogen: 119D5-F1 is a mouse monoclonal IgG1/kappa antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.

Clone: 119D5-F1

Isotype: IgG1

Application Details

Application Notes: 119D5-F1 reacts with an epitope located C-terminal of residue 231 in lamin B1. 119D5-F1 is suitable for immunocytochemistry, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Lamin B1 antibody (ABIN335391)

Broers, Ramaekers, Bonne, Yaou, Hutchison: "Nuclear lamins: laminopathies and their role in premature ageing." in: Physiological reviews, Vol. 86, Issue 3, pp. 967-1008, (2006) (PubMed).

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, (2003) (PubMed).

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, (1999) (PubMed).

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, (1997) (PubMed).

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, (1997) (PubMed).

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, (1997) (PubMed).

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, (1997) (PubMed).

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, (1996) (PubMed).

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, (1996) (PubMed).

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin B1 (LMNB1 Products)

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Pathways: Apoptosis, Caspase Cascade in Apoptosis