OCT4 antibody (AA 100-200)

Catalog No. ABIN336287

The Rabbit Polyclonal anti-OCT4 antibody has been validated for WB. It is suitable to detect OCT4 in samples from Human, Mouse and Protozoa.

Quick Overview for OCT4 antibody (AA 100-200) (ABIN336287)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Protozoa

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OCT4 Antibody

Binding Specificity: AA 100-200

Cross-Reactivity: Human, Mouse (Murine), Primate

Purification: affinity purified

Immunogen: A synthetic peptide made to an internal portion of the human OCT4 protein sequence (between AA 100-200).

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: OCT-4

Background: Oct4, also known as POU domain transcription factor, Octomerbinding transcription factor 3 and Oct3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct4, which disappears rapidly when the cells differentiate. Most studies on Oct4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct4 expression, the protein appears in the nucleus, implying biological activity. Functional Oct4 has been found in human amniotic fluid. As Oct4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct4 proteins within a cell. Oct4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation

Pathways: Stem Cell Maintenance