GJC1 antibody (AA 354-367)

Catalog No. ABIN337289

The Mouse Monoclonal anti-GJC1 antibody has been validated for WB, IHC, IHC (p) and ICC. It is suitable to detect GJC1 in samples from Human, Rat, Mouse and Dog.

Quick Overview for GJC1 antibody (AA 354-367) (ABIN337289)

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-GJC1 Antibody

Binding Specificity: AA 354-367

Brand: IHC-plus™

Specificity: Recognizes human Connexin 45. Species cross-reactivity: rat. Species sequence homology: canine: 100 % and mouse: 92 %.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa354-367 of human connexin 45. (H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu- Cys-OH) (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GJC1

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Alternative Name: GJC1 / CX45 / Connexin 45

Background: Name/Gene ID: GJC1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJC1, Connexin-45, Connexin 45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, CX45, Gap junction protein alpha-6, GJA7

Gene ID: 10052

UniProt: P36383

Pathways: Cell-Cell Junction Organization