ITPR1 antibody (AA 1892-1911)

Catalog No. ABIN337359

This anti-ITPR1 antibody is a Rabbit Polyclonal antibody detecting ITPR1 in IHC, WB, IP, ICC, IHC (p) and IHC (fro). Suitable for Human, Rat, Mouse, Cow and Monkey.

Quick Overview for ITPR1 antibody (AA 1892-1911) (ABIN337359)

Target: ITPR1 (Inositol 1,4,5-Trisphosphate Receptor, Type 1 (ITPR1))

Reactivity: Human, Rat, Mouse, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITPR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ITPR1 Antibody

Binding Specificity: AA 1892-1911

Brand: IHC-plus™

Specificity: Recognizes human IP3R-I. Detects a band of ~240kD by Western Blot. Species cross-reactivity: mouse, rat and canine.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine (100%) Hamster, Elephant, Panda, Horse (95%) Opossum (85%).

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to aa 1892-1911 (N1892KKKDDEVDRDAPSRKKAKE1911) of the cytoplasmic C-terminal domain of human IP3R-I (inositol 1,4,5-triphosphate receptor (type-I)). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine (100%), Hamster, Elephant, Panda, Horse (95%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr (1:125), IHC-P (5 μg/mL), IP, WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ITPR1

Target: ITPR1 (Inositol 1,4,5-Trisphosphate Receptor, Type 1 (ITPR1))

Alternative Name: ITPR1 / IP3 Receptor Type 1

Background: Name/Gene ID: ITPR1
Subfamily: Inositol 1,4,5-triphosphate receptor
Family: Ion Channel

Synonyms: ITPR1, InsP3R-I, IP3R1, InsP3R, InsP3R type I, INSP3R1, IP3R, Opisthotonus, Pcp1, IP3 receptor, IP3 receptor isoform 1, SCA15, Spinocerebellar ataxia 15, Spinocerebellar ataxia 16, I145TR, IP3R 1, KIAA1754, SCA16, Type 1 InsP3 receptor

Gene ID: 3708

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, Synaptic Membrane, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma