CD3 antibody

Catalog No. ABIN340660

This Mouse Monoclonal antibody specifically detects CD3 in FACS and Func. It exhibits reactivity toward Monkey.

Quick Overview for CD3 antibody (ABIN340660)

Target: CD3

Reactivity: Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Product Details anti-CD3 Antibody

Specificity: Recognizes rhesus monkey RhT3 antigen (homologue of the human CD3 antigen) with a molecular mass of 20-25kD. Reacts with CD3 from Macaca mulatta (rhesus monkey), Macaca arctoides (stumptail monkey), Macaca fascicularis (cynomolgus monkey), Cercocebus atys (sooty mangabey), Cercopithecus aethiops (African green monkey), and Papio anubis (baboon). Does not react with Callithrix jacobus (marmoset) or with any of the Hominoidea tested (human, chimpanzee, gorilla, and orangutan).

Purification: Ion exchange chromatography

Immunogen: Rhesus monkey peripheral blood leukocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Func

Usage: Suitable for use in flow cytometry. Use approximately 50 ng to label 1 x 1 x10⁶. In mitogenic studies, use approximately 1 μg/mL. For inhibition of Con A/PMA induced proliferation, use approximately 10 μg/mL. Myeloma/Fusion Partners: BALB/c mouse spleen cells were fused with SP2/O Ag14 mouse myeloma cells.

Comment:

Target Species of Antibody: Monkey

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 500 μL sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4, 125 mM trehalose

Storage: -20 °C

Storage Comment: Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway