CD14 antibody (CD14 Molecule)

Details for Product anti-CD14 Antibody No. ABIN343180
Antigen
Reactivity
Mouse (Murine)
732
110
51
43
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41
39
36
31
28
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21
16
15
13
7
7
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5
3
2
2
1
Host
Mouse
650
146
33
9
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD14 antibody is un-conjugated
95
81
63
53
16
10
10
8
7
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
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3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA
644
215
161
148
124
120
45
41
35
19
13
13
11
10
10
3
3
3
3
3
2
2
2
1
1
1
1
1
Options
Immunogen Monocytes of mouse
Clone BiG53
Isotype IgG1
Specificity Membrane bound and soluble mouse CD14. The antibody blocks LPS binding to membrane bound CD14.
Purification Protein A affinity column
Antigen
Alternative Name CD14 (CD14 Antibody Abstract)
Background Background: biG 53 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.
Gene ID 12475
NCBI Accession NP_033971
UniProt P10810
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Application Notes Suitable for immunostaining of CD14 positive cells and inhibition studies Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 ELISA. Inhibition of LPS binding to CD14 (Binding at LPS binding site). Up to 1 ug/ml biG53 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with bidistilled water
Concentration 1 mg/mL
Buffer PBS, pH 7.16
Preservative Without preservative
Storage -20 °C,-80 °C