AGR3 antibody

Catalog No. ABIN343711

This Mouse Monoclonal antibody specifically detects AGR3 in WB, IHC (p), IHC (fro) and ICC. It exhibits reactivity toward Human.

Quick Overview for AGR3 antibody (ABIN343711)

Target: AGR3 (Anterior Gradient 3 (AGR3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: AGR3-1

Product Details anti-AGR3 Antibody

Purpose: Anti-AGR3 Purified

Specificity: The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa), a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.

Cross-Reactivity (Details): Human, Other species Not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human AGR3 protein

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 1 μg/mL, positive tissue: breast cancer.
Immunohistochemistry (frozen sections): Recommended dilution: 1 μg/mL, positive tissue: breast cancer.
Western blotting: Recommended dilution: 1 μg/mL, positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/mL, positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for AGR3

Target: AGR3 (Anterior Gradient 3 (AGR3))

Alternative Name: AGR3

Background: Anterior gradient 3, protein disulphide isomerase,AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.,AG3, HAG3

Gene ID: 155465

UniProt: Q8TD06