alpha Actinin 4 antibody (N-Term)

Catalog No. ABIN347113

The Rabbit Polyclonal anti-alpha Actinin 4 antibody has been validated for WB, ELISA and IHC. It is suitable to detect alpha Actinin 4 in samples from Human.

Quick Overview for alpha Actinin 4 antibody (N-Term) (ABIN347113)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-alpha Actinin 4 Antibody

Binding Specificity: N-Term

Specificity: Reacts with human 110 kDa of ACTN4 protein

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with rat and mouse protein due to sequence homology.

Purification: Antiserum

Immunogen: Synthetic peptide derived from N- term domain of the human ACTN4 protein.

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC : 1/100 to 1/500 - WB : 1/500 to 1/2 000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: alpha-Actinin-4 (ACTN4)

Background: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions.

Gene ID: 81

UniProt: O43707

Pathways: Proton Transport