ATG4C antibody

Catalog No. ABIN349989

This anti-ATG4C antibody is a Sheep Polyclonal antibody detecting ATG4C in WB and IHC. Suitable for Human.

Quick Overview for ATG4C antibody (ABIN349989)

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This ATG4C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG4C Antibody

Purpose: Sheep antibody to ATG4C

Specificity: Specific for ATG4C.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human cysteine protease ATG4C (autophagin-3 APG4C AUTL1 AUTL3) conjugated to an immunogenic carrier protein has been used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for ATG4C

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Alternative Name: ATG4C

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation metamorphosis non-apoptotic cell death and aging. Reduced levels of autophagy have been described in some malignant tumors and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants encoding the same protein have been characterized. Cysteine protease required for autophagy which cleaves the C-terminal part of either MAP1LC3 GABARAPL2 or GABARAP allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II with a revealed C-terminal glycine is considered to be the phosphatidylethanolamine (PE)-conjugated form and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. TISSUE SPECIFICITY: Highly expressed in skeletal muscle heart liver and testis.

UniProt: Q96DT6

Pathways: Autophagy