Dynactin 1 antibody (AA 120-170)

Catalog No. ABIN7698974

The Sheep Polyclonal anti-Dynactin 1 antibody has been validated for WB and IHC. It is suitable to detect Dynactin 1 in samples from Human.

Quick Overview for Dynactin 1 antibody (AA 120-170) (ABIN7698974)

Target: Dynactin 1 (DCTN1)

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Dynactin 1 Antibody

Binding Specificity: AA 120-170

Purpose: Sheep antibody to DCTN1

Specificity: Specific for DCTN1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 120-170 of human DCTN1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and moue.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: DCTN1

Background: FUNCTION: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. SUBUNIT: Large macromolecular complex of at least 10 components, p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1 MAPRE2 and MAPRE3. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm cytoskeleton. TISSUE SPECIFICITY: Brain. DISEASE: Defects in DCTN1 are the cause of progressive lower motor neuron disease. PLMND is a progressive dominant disease that has no sensory symptoms.

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling