SLC1A1 antibody (Extracellular Domain)

Catalog No. ABIN350316

Product Details anti-SLC1A1 Antibody

Target: SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))

Binding Specificity: AA 150-200, Extracellular Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit antibody to SLC1A1 (150-200)

Specificity: Specific for EAAT3.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from extracellular domain of human EAAT3 (AA 150-200) conjugated to blue carrier protein was used as the antigen. The peptide shares 96% identity with rat and mouse sequence.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SLC1A1

Target: SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))

Alternative Name: SLC1A1 (SLC1A1 Products)

Background: FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Defects in SLC1A1 may be a cause of dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. This is as defect in renal and probably intestinal transport of glutamic and aspartic acids and is associated with moderate hyperprolinemia. SUBCELLULAR LOCATION: Membrane, Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in all tissues tested including liver muscle testis ovary retinoblastoma cell line neurons and brain (in which there was dense expression in substantia nigra red nucleus hippocampus and in cerebral cortical layers).

UniProt: P43005

Pathways: Dicarboxylic Acid Transport