Sortilin 1 antibody

Catalog No. ABIN350868

This anti-Sortilin 1 antibody is a Rabbit Polyclonal antibody detecting Sortilin 1 in WB and IHC. Suitable for Rat.

Quick Overview for Sortilin 1 antibody (ABIN350868)

Target: Sortilin 1 (SORT1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sortilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Sortilin 1 Antibody

Purpose: Rabbit antibody to Sortilin

Specificity: Specific for Sortilin.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from rat Sortilin conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Sortilin 1

Target: Sortilin 1 (SORT1)

Alternative Name: Sortilin

Background: FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP and GM2A LPL PSAP and SLC2A4. Forms a complex with NGFR which binds specifically to the precursor form of NGFB (proNGFB). Interacts with the precursor form of BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane, Single-pass type I membrane protein. Note: Localized to membranes of the endosomes Golgi stack lysosomes and nucleus. Localization to the plasma membrane in adipocytes is enhanced by insulin. Localized to the endoplasmic reticulum and the plasma membrane. Also found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. TISSUE SPECIFICITY: Highly expressed in fat brain and lung. Expressed in neuronal bodies and dendrites of the piriform cortex and hippocampus. Also expressed in the islands of Calleja medial and lateral septal nuclei amygdaloid nuclei thalamic nuclei the supraoptic nucleus the substantia nigra the Purkinje layer of the cerebellar cortex and the cranial motor nerve nuclei of the brainstem.

UniProt: O54861

Pathways: Neurotrophin Signaling Pathway