TRPV1 antibody (4th Cytoplasmic Loop)

Catalog No. ABIN351248

Product Details anti-TRPV1 Antibody

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Binding Specificity: 4th Cytoplasmic Loop

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This TRPV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Sheep antibody to TRPV1

Specificity: Specific for TRPV1.

Cross-Reactivity: Guinea Pig, Human

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide the 4th cytoplasmic loop of human TRPV1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in guinea pig.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for TRPV1

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Alternative Name: TRPV1 (TRPV1 Products)

Background: Capsaicin the main pungent ingredient in hot chili peppers elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range suggesting that it functions as a transducer of painful thermal stimuli in vivo. FUNCTION: Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases which involves PKC isozymes and PCL. Responses evoked by low pH and heat and capsaicin can be antagonized by capsazepine.

Pathways: Dicarboxylic Acid Transport