TRPV4 antibody (Cytoplasmic Loop)

Catalog No. ABIN351284

Product Details anti-TRPV4 Antibody

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Binding Specificity: Cytoplasmic Loop

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This TRPV4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Sheep antibody to TRPV4

Specificity: Specific for TRPV4.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the 1t cytoplasmic loop of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and human.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for TRPV4

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Alternative Name: TRPV4 (TRPV4 Products)

Background: TRPV4 is a cation-selective channel activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN SRC FYN HCK LCK and YES. SUBCELLULAR LOCATION: Membrane, Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung spleen kidney testis fat and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

UniProt: Q9EPK8

Pathways: Hormone Transport, Cell-Cell Junction Organization