PPIF antibody (AA 30-207)

Catalog No. ABIN356236

This Mouse Monoclonal antibody specifically detects PPIF in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for PPIF antibody (AA 30-207) (ABIN356236)

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: AT1F5

Product Details anti-PPIF Antibody

Binding Specificity: AA 30-207

Specificity: The antibody recognizes PPIF.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: PPIase F, Rotamase F, PPIF, CYP3, mitochondrial Peptidyl-prolyl cis-trans isomerase

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human PPIF (30-207 aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (1: 500 - 1: 1,000). Immunohistochemistry on paraffin sections (1: 50 - 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PPIF

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Alternative Name: Cyclophilin F

Background: Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-transisomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.Synonyms: CYP3, PPIF, PPIase F, Rotamase F, mitochondrial Peptidyl-prolyl cis-trans isomerase

Gene ID: 10105

UniProt: P30405

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity