CD175s antibody

Catalog No. ABIN356328

This anti- antibody is a Mouse Monoclonal antibody detecting in FACS, IHC (fro), IF and IHC (p). Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD175s antibody (ABIN356328)

Target: CD175s

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: STN218

Product Details

Characteristics: Synonyms: Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn, NeuAc a GalNAc

Purification: Purified

Immunogen: Ovine submaxillary mucin (OSM)Remarks: Molecular weight of antigen: Several HM weight glycoproteins. Epitope: NeuAc alphaGalNacOSer/Thr.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence. Flow Cytometry. Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

References

Hofmann, Schlüter, Lange, Mercanoglu, Ewald, Fölster, Picksak, Harder, El Gammal, Grupp, Güngör, Drenckhan, Schlüter, Wagener, Izbicki, Jücker, Bockhorn, Wolters-Eisfeld: "COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer." in: Molecular cancer, Vol. 14, pp. 109, (2015) (PubMed).

Target Details

Target: CD175s

Background: Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.Synonyms: NeuAc a GalNAc, Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn