Thyroglobulin antibody (FITC)

Catalog No. ABIN356333

This Mouse Monoclonal antibody specifically detects Thyroglobulin in FACS, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Thyroglobulin antibody (FITC) (ABIN356333)

Target: Thyroglobulin (TG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: TG221

Product Details anti-Thyroglobulin Antibody (FITC)

Specificity: This antibody Tg is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of Tg in metastatic lesion establishes thyroid origin of tumor.
Cellular Localization: Cytoplasmic.

Characteristics: Synonyms: Thyreoglobulin, TGN

Immunogen: Purified human Thyroglobulin. Remarks: Molecular weight of antigen: 330 kDa.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or itcan also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL(1/200-1/1,000). Recommended Positive Control: Human thyroid.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Background: Thyroglobulin (Tg) is a 660 kDa dimeric protein produced by and used by entirely within thyroid gland. Tg is used by the thyroid gland to produce the thyroid hormone thyroxine (T4) and triiodothyronine (T3). Tg is produced by the thyroid epithelial cells, called thyrocytes, which form spherical follicles. Tg is stored in the follicle lumen. Tg levels in the blood can be used as markers for certain kind of thyroid cancer. Tg levels are also elevated in Graves disease.Synonyms: TGN, Thyreoglobulin

Gene ID: 7038

UniProt: P01266

Pathways: Thyroid Hormone Synthesis