EIF4B antibody

Catalog No. ABIN357289

This Rabbit Polyclonal antibody specifically detects EIF4B in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for EIF4B antibody (ABIN357289)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EIF4B Antibody

Specificity: This antibody detects EIF4B.

Purification: Protein A column, followed by peptide affinity purification

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S422 of human EIF4B.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: EIF4B

Background: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B

Molecular Weight: 69151 Da

Gene ID: 1975, 9606

UniProt: P23588