CD73 antibody (N-Term)

Catalog No. ABIN357389

The Rabbit Polyclonal anti-CD73 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect CD73 in samples from Human.

Quick Overview for CD73 antibody (N-Term) (ABIN357389)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CD73 Antibody

Binding Specificity: AA 1-30, N-Term

Specificity: This antibody detects CD73 (NT5E) at N-term.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human NT5E . Genename: NT5E

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.Synonyms: 5'-nucleotidase, NT5, NT5E, NTE

Gene ID: 4907, 9606

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process