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CD73 antibody (C-Term)

This anti-CD73 antibody is a Rabbit Polyclonal antibody detecting CD73 in WB, IHC (p) and EIA. Suitable for Human and Mouse.
Catalog No. ABIN357390

Quick Overview for CD73 antibody (C-Term) (ABIN357390)

Target

See all CD73 (NT5E) Antibodies
CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity

  • 177
  • 69
  • 36
  • 19
  • 18
  • 16
  • 4
  • 2
  • 1
  • 1
  • 1
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  • 1
Human, Mouse

Host

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  • 90
  • 14
  • 2
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  • 1
Rabbit

Clonality

  • 121
  • 94
  • 1
Polyclonal

Conjugate

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  • 7
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  • 5
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  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CD73 antibody is un-conjugated

Application

  • 150
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  • 55
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  • 5
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  • 1
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 16
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    • 9
    • 9
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    • 5
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    • 1
    • 1
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    C-Term

    Specificity

    This antibody detects CD73 (C-term).

    Purification

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

    Alternative Name

    CD73

    Background

    Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.Synonyms: 5'-nucleotidase, NT5, NT5E, NTE

    Molecular Weight

    63367 Da

    Gene ID

    4907, 9606

    UniProt

    P21589

    Pathways

    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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