UCHL5 antibody (N-Term)

Catalog No. ABIN357513

This anti-UCHL5 antibody is a Rabbit Polyclonal antibody detecting UCHL5 in WB, EIA and IHC (p). Suitable for Human and Mouse.

Quick Overview for UCHL5 antibody (N-Term) (ABIN357513)

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UCHL5 Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific to UCHL5 (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UCHL5.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for UCHL5

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Alternative Name: UCHL5

Background: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.Synonyms: AD-019, CGI-70, UCH-L5, UCH37, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin thioesterase L5

Molecular Weight: 37641 Da

Gene ID: 51377, 9606

UniProt: Q9Y5K5