ART3 antibody (C-Term)

Catalog No. ABIN357664

This Rabbit Polyclonal antibody specifically detects ART3 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for ART3 antibody (C-Term) (ABIN357664)

Target: ART3 (ADP-Ribosyltransferase 3 (ART3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ART3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ART3 Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific to ART3/TMART (C-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ART3.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ART3

Target: ART3 (ADP-Ribosyltransferase 3 (ART3))

Background: Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35 % amino acid identity.Synonyms: Ecto-ADP-ribosyltransferase 3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)-arginine ADP-ribosyltransferase 3

Molecular Weight: 43923 Da

Gene ID: 419, 9606

UniProt: Q13508