BIK antibody (pThr33)

Catalog No. ABIN358090

This Rabbit Polyclonal antibody specifically detects BIK in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for BIK antibody (pThr33) (ABIN358090)

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-BIK Antibody

Binding Specificity: pThr33

Specificity: This antibody detects BIK pThr33.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T33 of human Bik.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for BIK

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Alternative Name: BIK

Background: The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.Synonyms: Apoptosis inducer NBK, BIP1, BP4, Bcl-2-interacting killer, NBK

Molecular Weight: 18016 Da

Gene ID: 638, 9606

UniProt: Q13323

Pathways: Positive Regulation of Endopeptidase Activity