MAP4K1 antibody (pThr165)

Catalog No. ABIN358384

This Rabbit Polyclonal antibody specifically detects MAP4K1 in EIA. It exhibits reactivity toward Human.

Quick Overview for MAP4K1 antibody (pThr165) (ABIN358384)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-MAP4K1 Antibody

Binding Specificity: pThr165

Specificity: This antibody detects MAP4K1 pThr165. Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T165 of human MAP4K1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: MAP4K1

Background: The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.Synonyms: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1

Molecular Weight: 91166 Da

Gene ID: 11184, 9606

UniProt: Q92918

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor