PNPLA3 antibody (Internal Region)

Catalog No. ABIN361184

The Goat Polyclonal anti-PNPLA3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect PNPLA3 in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for PNPLA3 antibody (Internal Region) (ABIN361184)

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PNPLA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PNPLA3 Antibody

Binding Specificity: Internal Region

Purpose: Pnpla3 / Adiponutrin (mouse)

Sequence: EHDICPKVKS TN

Specificity: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 8 μg/mL.

Western Blot: Approx 45 kDa band observed in Mouse Adrenal Gland lysates, and also in preliminary testing of Mouse and Human Kidney and Rat Adrenal Gland lysate (calculated MW of 42.6Da according to Mouse NP_473429.2). Recommended concentration: 0.01-0.03

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PNPLA3 antibody (ABIN361184)

Yang, Mottillo, Mladenovic-Lucas, Zhou, Granneman: "Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes." in: Nature metabolism, Vol. 1, Issue 5, pp. 560-569, (2019) (PubMed).

Hao, Ito, Huang, Sae-tan, Lambert, Ross: "Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells." in: Metabolism: clinical and experimental, Vol. 63, Issue 10, pp. 1352-62, (2014) (PubMed).

Kumari, Schoiswohl, Chitraju, Paar, Cornaciu, Rangrez, Wongsiriroj, Nagy, Ivanova, Scott, Knittelfelder, Rechberger, Birner-Gruenberger, Eder, Brown, Haemmerle, Oberer, Lass, Kershaw, Zimmermann et al.: "Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase. ..." in: Cell metabolism, Vol. 15, Issue 5, pp. 691-702, (2012) (PubMed).

Gustavsson, Soga, Wahlström, Vesterlund, Azimi, Norstedt, Tollet-Egnell: "Sex-dependent hepatic transcripts and metabolites in the development of glucose intolerance and insulin resistance in Zucker diabetic fatty rats." in: Journal of molecular endocrinology, Vol. 47, Issue 2, pp. 129-43, (2011) (PubMed).

Target Details for PNPLA3

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Alternative Name: Pnpla3

Background: Pnpla3, patatin-like phospholipase domain containing 3, Adpn, adiponutrin

Molecular Weight: 42.6kDa

Gene ID: 80339, 116939, 362972

NCBI Accession: NP_473429