NEFH antibody

Catalog No. ABIN361350

This anti-NEFH antibody is a Mouse Monoclonal antibody detecting NEFH in WB and IF. Suitable for Cow.

Quick Overview for NEFH antibody (ABIN361350)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: NAP4

Product Details anti-NEFH Antibody

Specificity: Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Cross-Reactivity: Chicken, Mammalian

Purification: Protein G purified culture supernatant

Immunogen: Purified bovine NF-H

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: WB: 1:5,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NEFH

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.

Molecular Weight: '200 kDa

Gene ID: 4744

UniProt: P12036